Moisture can enter the duct system through leaks or if the system has been improperly installed or serviced. Research suggests that condensation (which occurs when a surface temperature is lower than the dew point temperature of the surrounding air) on or near cooling coils of air conditioning units is a major factor in moisture contamination of the system. The presence of condensation or high relative humidity is an important indicator of the potential for mold growth on any type of duct. Controlling moisture can often be difficult, but here are some steps you can take:
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Internal insulation provides better acoustical (noise) control. Flexible duct is very low cost. These products are engineered specifically for use in ducts or as ducts themselves, and are tested in accordance with standards established by Underwriters Laboratories (UL), the American Society for Testing and Materials (ASTM), and the National Fire Protection Association (NFPA). Many insulated duct systems have operated for years without supporting significant mold growth. Keeping them reasonably clean and dry is generally adequate. However, there is substantial debate about whether porous insulation materials (e.g., fiber glass) are more prone to microbial contamination than bare sheet metal ducts. If enough dirt and moisture are permitted to enter the duct system, there may be no significant difference in the rate or extent of microbial growth in internally lined or bare sheet metal ducts. However, treatment of mold contamination on bare sheet metal is much easier. Cleaning and treatment with an EPA-registered biocide are possible. Once fiberglass duct liner is contaminated with mold, cleaning is not sufficient to prevent re-growth and there are no EPA-registered biocides for the treatment of porous duct materials. EPA, NADCA and NAIMA all recommend the replacement of wet or moldy fiber glass duct material.

You should be aware that although a substance may look like mold, a positive determination of whether it is mold or not can be made only by an expert and may require laboratory analysis for final confirmation. For about $50, some microbiology laboratories can tell you whether a sample sent to them on a clear strip of sticky household tape is mold or simply a substance that resembles it.

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